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Recombinant YTHDF2 (401-554) protein

Expressed In

E. coli

Protein Species

Human

Cat No : 81103,81803 81103

验证数据展示

  • Recombinant YTHDF2 (401-554) SDS PAGE gel 12.5% SDS-PAGE Coomassie staining MW: 22.7 kDa Purity: >85%

    Recombinant YTHDF2 (401-554) SDS PAGE gel 12.5% SDS-PAGE Coomassie staining MW: 22.7 kDa Purity: >85%

  • HTRF for YTHDF2 (401-554) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 (401-554) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF for YTHDF2 (401-554) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 (401-554) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

  • HTRF for YTHDF2 (401-554) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 (401-554) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF for YTHDF2 (401-554) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 (401-554) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

产品信息

Expressed In E. coli
Protein Species Human

Contents

Recombinant YTHDF2 (401-554) protein is supplied in 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 20% glycerol, 2 mM DTT, and 0.04% Triton X-100.

背景介绍

YTHDF2 (YT521-B homology domain family 2, also known as CAHL, HGRG8, NY-REN-2) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF proteins contain a highly conserved single-stranded RNA-binding domain which can specifically recognize and bind m6A-containing RNAs. An important role of YTHDF2 is in regulating mRNA stability. YTHDF2 recognizes many m6A-containing mRNAs that are not being actively translated and recruits them to cytoplasmic processing (P) bodies for degradation. YTHDF2 preserves 5′UTR methylation of stress- induced transcripts and promotes cap-independent mRNA translation by competitive binding and limiting the m6A ‘eraser’ FTO from demethylation. N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).

Application Notes

Recombinant YTHDF2 (401-554) protein is suitable for use in the study of binding assay, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant YTHDF2 (401-554) protein corresponding to amino acids 401-554 that contains the YTH domain sequence of human YTHDF2 (accession number NP_057342.2) was expressed in E. coli cells with an N-terminal 6xHis Tag and a C-terminal Flag tag. The molecular weight of the protein is 22.7 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.