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Recombinant YTHDF3 (407-560) protein

Expressed In

E. coli

Protein Species

Human

Cat No : 81104,81804 81104

验证数据展示

  • Recombinant YTHDF3 (407-560) SDS PAGE gel 12.5% SDS-PAGE gel with Coomassie Bluestaining MW: 22.6 kDa Purity: >80%

    Recombinant YTHDF3 (407-560) SDS PAGE gel 12.5% SDS-PAGE gel with Coomassie Bluestaining MW: 22.6 kDa Purity: >80%

  • HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

  • HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul FLAG antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

产品信息

Expressed In E. coli
Protein Species Human

Contents

Recombinant YTHDF3 (407-560) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20% glycerol, 2 mM DTT, 0.04% Triton X-100.

背景介绍

YTHDF3 (YTH N6-Methyladenosine RNA Binding Protein 3) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs and promotes RNA translation efficiency, sharing m6A-containing mRNAs targets with YTHDF1 and YTHDF2, and regulates different processes depending on the context. It facilitates the translation of targeted mRNAs in cooperation with YTHDF1 by binding to m6A-containing mRNAs and interacting with 40S and 60S ribosome subunits. It can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation. YTHDF3 recognizes and binds m6A-containing circular RNAs (circRNAs) and promotes their translation. circRNAs are generated through back-splicing of pre-mRNAs, a non-canonical splicing process promoted by dsRNA structures across circularizing exons. N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messenger RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).

Application Notes

Recombinant YTHDF3 (407-560) protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant human YTHDF3 (407-560) protein corresponding to amino acids 407-560 that contains the YTH domain sequence of human YTHDF3 (accession number NP_689971.4) was expressed in E. coli cells with an N-terminal 6xHis Tag and a C-terminal Flag tag. The molecular weight of the protein is 22.6 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.