Recombinant beta-Glucosyltransferase protein

Recombinant beta-Glucosyltransferase protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, and 0.5 mM TCEP.

Beta-Glucosyltransferase, also called as b-Glucosyltransferase or BGT, is a DNA-modifying enzyme. It is encoded by a number of bacteriophages belonging to the T-even group. It catalyses the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. BGT shows little DNA sequence specificity but modify only 5-HMC bases. The glucosylation protects the infecting viral DNA from host nucleases. In addition to its protective role, the glucosylation has also been implicated in the control of phage-specific gene expression by influencing transcription in vivo and in vitro. The reactions is: UDP-Glc + 5-HMC-DNA → b-glucosyl- HMC-DNA + UDP.

Recombinant beta-Glucosyltransferase protein is useful for the study of glucosyltransferase assays in vitro.

Beta-Glucosyltransferase was expressed in E. coli cells as the full length protein (accession number NP_049658.1) with an N-terminal 6xHis Tag. The molecular weight of beta-Glucosyltransferase protein is 42.8 kDa.

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.