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验证数据展示

  • WB analysis using 30182-1-AP

    WB analysis using 30182-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30182-1-AP (Ribosomal protein L4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30182-1-AP

    WB analysis of HeLa using 30182-1-AP

    WB result of Ribosomal protein L4 antibody (30182-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HeLa cells.

  • IHC staining of human stomach cancer using 30182-1-AP

    IHC staining of human stomach cancer using 30182-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30182-1-AP (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL4 antibody included
  • IF Staining of HepG2 using 30182-1-AP

    IF Staining of HepG2 using 30182-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ribosomal protein L4 antibody (30182-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

Ribosomal protein L4 Polyclonal antibody

Ribosomal protein L4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30182-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30182-1-AP targets Ribosomal protein L4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Ribosomal protein L4 fusion protein Ag32272 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L4
别名 RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1
计算分子量 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC009888
基因名称 Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_3086255
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36578
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

实验方案

Product Specific Protocols
WB protocol for Ribosomal protein L4 antibody 30182-1-APDownload protocol
IHC protocol for Ribosomal protein L4 antibody 30182-1-APDownload protocol
IF protocol for Ribosomal protein L4 antibody 30182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols