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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

    WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80959-1-RR (RPL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

    IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

    IP result of anti-Ribosomal protein L4 (IP:80959-1-RR, 4ug; Detection:80959-1-RR 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 80959-1-RR (same clone as 80959-1-PBS)

    IHC staining of human stomach cancer using 80959-1-RR (same clone as 80959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPL4 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR (same clone as 80959-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR (same clone as 80959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPL4 antibody included
  • FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (80959-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

Ribosomal protein L4 Recombinant antibody, PBS Only

Ribosomal protein L4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 80959-1-PBS

产品说明书

CloneNo. 3K6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.

产品信息

80959-1-PBS targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 Ribosomal protein L4 fusion protein Ag28728 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L4
别名 RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC009888
基因名称 Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_2918922
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36578
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.