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Ribosomal protein L4 Recombinant antibody, PBS Only

Ribosomal protein L4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K6

Cat no : 80959-1-PBS

Print datasheet

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

验证数据展示

  • WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

    WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80959-1-RR (RPL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

    IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

    IP result of anti-Ribosomal protein L4 (IP:80959-1-RR, 4ug; Detection:80959-1-RR 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (80959-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.

产品信息

80959-1-PBS targets Ribosomal protein L4 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Ribosomal protein L4 fusion protein Ag28728 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein L4
Synonyms RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC009888
Gene Symbol Ribosomal protein L4
Gene ID (NCBI) 6124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36578
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.