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验证数据展示

  • WB analysis of human heart using 16027-1-AP

    WB analysis of human heart using 16027-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human malignant melanoma using 16027-1-AP

    IHC staining of human malignant melanoma using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens)..

    Ready-to-use IHC kits available with S100A1 antibody included
  • IHC staining of human malignant melanoma using 16027-1-AP

    IHC staining of human malignant melanoma using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens)..

    Ready-to-use IHC kits available with S100A1 antibody included
  • IF Staining of human ovary cancer using 16027-1-AP

    IF Staining of human ovary cancer using 16027-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human ovary cancer using 16027-1-AP

    IF Staining of human ovary cancer using 16027-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

S100A1 Polyclonal antibody

S100A1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 16027-1-AP

产品说明书

引用文献(8)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

别名

Protein S100 A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100, S100 alpha, S100A, S100A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman malignant melanoma tissue, human kidney tissue, human ovary tumor tissue, human appendicitis tissue, human tonsillitis tissue, rat brain tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary cancer tissue
Planning an IHC experiment? We recommend our IHCeasy S100A1 Ready-To-Use IHC Kit. S100A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16027-1-AP targets S100A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 S100A1 fusion protein Ag8871 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A1
别名 Protein S100 A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100, S100 alpha, S100A, S100A1
计算分子量 94 aa, 11 kDa
观测分子量 10 kDa
GenBank蛋白编号BC014392
基因名称 S100A1
Gene ID (NCBI) 6271
RRIDAB_2183214
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23297
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for S100A1 antibody 16027-1-APDownload protocol
IHC protocol for S100A1 antibody 16027-1-APDownload protocol
IF protocol for S100A1 antibody 16027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Biomaterials

Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.

Authors - Xianzhen Dong
View Article
humanIHC

Mol Neurobiol

Anti-Apoptotic Effect of IGF1 on Schwann Exposed to Hyperglycemia is Mediated by Neuritin, a Novel Neurotrophic Factor.

Authors - Lingfei Yan
View Article
ratWB

Front Pharmacol

Reynoutrin Improves Ischemic Heart Failure in Rats Via Targeting S100A1.

Authors - Wenkai Yang
  • KD Validated
View Article
humanWB

Circ Heart Fail

S100A1 in Human Heart Failure: Lack of Recovery Following LVAD Support.

Authors - Mosi K Bennett
View Article
ratWB,IF

Mol Med Rep

S100A1 expression is increased in spinal cord injury and promotes inflammation, oxidative stress and apoptosis of PC12 cells induced by LPS via ERK signaling

Authors - Ye Bai
View Article
ratIF

Sci Adv

An injectable and adaptable hydrogen sulfide delivery system for modulating neuroregenerative microenvironment

Authors - Xianzhen Dong
View Article