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S100A10 Polyclonal antibody

S100A10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 11250-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (3)

Calpactin-1 light chain, Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HT-29 cells, human lung tissue, HaCaT cells
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue, human gliomas tissue, human normal colon, human pancreas cancer tissue, human prostate hyperplasia tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy S100A10 Ready-To-Use IHC Kit. S100A10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11250-1-AP targets S100A10 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, FC, IP, ELISA
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 S100A10 fusion protein Ag1779 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A10
别名 Calpactin-1 light chain, Calpactin I light chain, Calpactin 1 light chain, CAL1L, ANX2LG
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC015973
基因名称 S100A10
Gene ID (NCBI) 6281
RRIDAB_2269906
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60903
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

实验方案

Product Specific Protocols
WB protocol for S100A10 antibody 11250-1-APDownload protocol
IHC protocol for S100A10 antibody 11250-1-APDownload protocol
IF protocol for S100A10 antibody 11250-1-APDownload protocol
FC protocol for S100A10 antibody 11250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection.

Authors - Yunwei Lou
mouseIF

Acta Pharmacol Sin

VEGFD/VEGFR3 signaling contributes to the dysfunction of the astrocyte IL-3/microglia IL-3Rα cross-talk and drives neuroinflammation in mouse ischemic stroke

Authors - Shuai Wang
mouseWB,IF

Int J Biol Macromol

Astrocyte-conditional knockout of MOB2 inhibits the phenotypic conversion of reactive astrocytes from A1 to A2 following spinal cord injury in mice

Authors - Xin Tao
ratIF

JCI Insight

Fabry disease Schwann cells release p11 to induce sensory neuron hyperactivity

Authors - Tyler B Waltz
mouseWB,IF

Phytomedicine

Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis

Authors - Zixian Zhou
mouseWB,IP

Diabetes

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Authors - Hua Qu
  • KD Validated
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