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  • Featured Product
  • KD/KO Validated

S100A13 Polyclonal antibody

S100A13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14987-1-AP

Print datasheet

Synonyms

S100 calcium-binding protein A13, Protein S100-A13, Protein S100 A13

验证数据展示

  • WB analysis of MCF-7 using 14987-1-AP

    WB analysis of MCF-7 using 14987-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14987-1-AP (S100A13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 14987-1-AP

    IHC staining of human thyroid cancer using 14987-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14987-1-AP (S100A13 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human thyroid cancer using 14987-1-AP

    IHC staining of human thyroid cancer using 14987-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14987-1-AP (S100A13 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 14987-1-AP

    IF Staining of MCF-7 using 14987-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14987-1-AP (S100A13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman thyroid cancer tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, human thyroid tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14987-1-AP targets S100A13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen S100A13 fusion protein Ag6971 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein A13
Synonyms S100 calcium-binding protein A13, Protein S100-A13, Protein S100 A13
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC000632
Gene Symbol S100A13
Gene ID (NCBI) 6284
RRIDAB_2878097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99584
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for S100A13 antibody 14987-1-APDownload protocol
IHC protocol for S100A13 antibody 14987-1-APDownload protocol
IF protocol for S100A13 antibody 14987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Gastroenterology

Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors

Authors - Mingjun Sun
View Article
humanIHC,WB

J Oral Pathol Med

Systematic screening identifies a TEAD4-S100A13 axis modulating cisplatin sensitivity of oral squamous cell carcinoma cells.

Authors - Ruicen Li
  • KD Validated
View Article
humanWB,IHC

Front Immunol

Identification of a novel immune-related gene signature for prognosis and the tumor microenvironment in patients with uveal melanoma combining single-cell and bulk sequencing data

Authors - Wanpeng Wang
  • KD Validated
View Article