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验证数据展示

  • WB analysis of HepG2 using 17924-1-AP

    WB analysis of HepG2 using 17924-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human meningioma using 17924-1-AP

    IHC staining of human meningioma using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human meningioma using 17924-1-AP

    IHC staining of human meningioma using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 17924-1-AP

    IF Staining of HepG2 using 17924-1-AP

    Immunofluorescent analysis of HepG2 cells using 17924-1-AP (S100A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

S100A5 Polyclonal antibody

S100A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17924-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

S100D, S100 calcium-binding protein A5, Protein S-100D, Protein S100-A5, Protein S100 A5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human kidney tissue, human liver tissue, SH-SY5Y cells
Positive IHC detected inhuman meningioma tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17924-1-AP targets S100A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 S100A5 fusion protein Ag12342 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A5
别名 S100D, S100 calcium-binding protein A5, Protein S-100D, Protein S100-A5, Protein S100 A5
计算分子量 110 aa, 13 kDa
观测分子量 11 kDa
GenBank蛋白编号BC093955
基因名称 S100A5
Gene ID (NCBI) 6276
RRIDAB_2183794
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33763
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for S100A5 antibody 17924-1-APDownload protocol
IHC protocol for S100A5 antibody 17924-1-APDownload protocol
IF protocol for S100A5 antibody 17924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Adv Sci (Weinh)

S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma

Authors - Huihuang Li
View Article
mouseIHC

Mol Cell Proteomics

The Membrane Proteome of Sensory Cilia to the Depth of Olfactory Receptors.

Authors - Katja Kuhlmann
View Article