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S100A6 Monoclonal antibody, PBS Only

S100A6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D11C8

Cat no : 66098-1-PBS

Print datasheet

Synonyms

MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY, CABP

验证数据展示

  • WB analysis of HeLa using 66098-1-Ig (same clone as 66098-1-PBS)

    WB analysis of HeLa using 66098-1-Ig (same clone as 66098-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A6 antibody included
  • IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A6 antibody included
  • IF Staining of HepG2 using 66098-1-Ig (same clone as 66098-1-PBS)

    IF Staining of HepG2 using 66098-1-Ig (same clone as 66098-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A6 antibody (66098-1-Ig, Clone: 3D11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy S100A6 Ready-To-Use IHC Kit. S100A6 primary antibody included.

产品信息

66098-1-PBS targets S100A6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen S100A6 fusion protein Ag17606 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name S100 calcium binding protein A6
Synonyms MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY, CABP
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC001431
Gene Symbol S100A6
Gene ID (NCBI) 6277
RRIDAB_2881497
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06703
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. Although S100A6 is a cytoplasmic protein, it can be located in the nuclear and cytoplasmic membranes in the presence of Ca2+ (PMID: 37670392).