验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human oesophagus cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13061-1-AP targets S100A7/Psoriasin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | S100A7/Psoriasin fusion protein Ag3738 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | S100 calcium binding protein A7 |
| 别名 | S100A7, S100 calcium-binding protein A7, Psoriasin, PSOR1, Protein S100-A7 |
| 计算分子量 | 101 aa, 11 kDa |
| 观测分子量 | 11 kDa |
| GenBank蛋白编号 | BC034687 |
| 基因名称 | S100A7/Psoriasin |
| Gene ID (NCBI) | 6278 |
| RRID | AB_2877908 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31151 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for S100A7/Psoriasin antibody 13061-1-AP | Download protocol |
| IHC protocol for S100A7/Psoriasin antibody 13061-1-AP | Download protocol |
| IF protocol for S100A7/Psoriasin antibody 13061-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Eur Acad Dermatol Venereol TOPK promotes the development of psoriasis and worenine alleviates psoriasiform dermatitis by inhibiting TOPK activity | ||
J Eur Acad Dermatol Venereol Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi. | ||
BMC Cancer A novel metabolic subtype with S100A7 high expression represents poor prognosis and immuno-suppressive tumor microenvironment in bladder cancer | ||
Cell Biol Int S100A7 promotes the development of human endometriosis by activating NF-κB signaling pathway in endometrial stromal cells. | ||
Oncol Lett PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma. | ||
Mol Med Rep TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes. |
