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验证数据展示

  • WB analysis of MCF-7 using 26656-1-AP

    WB analysis of MCF-7 using 26656-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-468 using 26656-1-AP

    WB analysis of MDA-MB-468 using 26656-1-AP

    MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 26656-1-AP

    IHC staining of human oesophagus cancer using 26656-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 26656-1-AP

    IHC staining of human oesophagus cancer using 26656-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

S100A7/Psoriasin Polyclonal antibody

S100A7/Psoriasin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26656-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Protein S100 A7, PSOR1, Psoriasin, S100A7, S100A7/Psoriasin, S100A7c

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-468 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26656-1-AP targets S100A7/Psoriasin in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 S100A7/Psoriasin fusion protein Ag24717 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A7
别名 Protein S100 A7, PSOR1, Psoriasin, S100A7, S100A7/Psoriasin, S100A7c
计算分子量 101 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC034687
基因名称 S100A7/Psoriasin
Gene ID (NCBI) 6278
RRIDAB_2880592
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31151
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.

实验方案

Product Specific Protocols
WB protocol for S100A7/Psoriasin antibody 26656-1-APDownload protocol
IHC protocol for S100A7/Psoriasin antibody 26656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols