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S100A9 Recombinant antibody

S100A9 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240539F4

Cat no : 83578-6-RR

Print datasheet

Synonyms

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

验证数据展示

  • IF Staining of RAW 264.7 using 83578-6-RR

    IF Staining of RAW 264.7 using 83578-6-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using S100A9 antibody (83578-6-RR, Clone: 240539F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 83578-6-RR

    FC experiment of A431 using 83578-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of Jurkat using 83578-6-RR

    FC experiment of Jurkat using 83578-6-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83578-6-RR

    Affinity and Kinetic Characterization of 83578-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-6-RR against Human S100A9 were performed. The affinity constant is 1.19 nM.

经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells
Positive FC (Intra) detected inA431 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy S100A9 Ready-To-Use IHC Kit. S100A9 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83578-6-RR targets S100A9 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen S100A9 fusion protein Ag25764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein A9
Synonyms CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

实验方案

Product Specific Protocols
IF protocol for S100A9 antibody 83578-6-RRDownload protocol
FC protocol for S100A9 antibody 83578-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols