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S100P Monoclonal antibody

S100P Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G6C3

Cat no : 67485-1-Ig

Print datasheet

Synonyms

MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P

验证数据展示

  • WB analysis of BXPC-3 using 67485-1-Ig

    WB analysis of BXPC-3 using 67485-1-Ig

    BXPC-3 cells were subjected to SDS PAGE followed by western blot with 67485-1-Ig (S100P antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67485-1-Ig

    IHC staining of human pancreas cancer using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100P antibody included
  • IHC staining of human pancreas cancer using 67485-1-Ig

    IHC staining of human pancreas cancer using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100P antibody included
  • IF Staining of HeLa using 67485-1-Ig

    IF Staining of HeLa using 67485-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100P antibody (67485-1-Ig, Clone: 1G6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inBXPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue, human placenta tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy S100P Ready-To-Use IHC Kit. S100P primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67485-1-Ig targets S100P in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen S100P fusion protein Ag19115 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name S100 calcium binding protein P
Synonyms MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P
Calculated Molecular Weight 95 aa, 11 kDa
Observed Molecular Weight 10 KDa
GenBank Accession NumberBC006819
Gene Symbol S100P
Gene ID (NCBI) 6286
RRIDAB_2882712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25815
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

实验方案

Product Specific Protocols
WB protocol for S100P antibody 67485-1-IgDownload protocol
IHC protocol for S100P antibody 67485-1-IgDownload protocol
IF protocol for S100P antibody 67485-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols