验证数据展示
经过测试的应用
Positive WB detected in | serum from mouse injected with bacteria tissue |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13192-1-AP targets SAA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | SAA fusion protein Ag3869 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | serum amyloid A2 |
别名 | SAA2, Amyloid A2 protein, serum amyloid A2, Serum amyloid A-2 protein |
计算分子量 | 122 aa, 14 kDa |
观测分子量 | 12-13 kDa |
GenBank蛋白编号 | BC020795 |
基因名称 | SAA2 |
Gene ID (NCBI) | 6289 |
RRID | AB_2877923 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P0DJI9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human SAA proteins are a group of apolipoproteins mainly found in the high density lipoprotein (HDL) portion of plasma. The serum amyloid A (SAA) protein is an acute phase apolipoprotein reactant produced mainly by hepatocytes and under the regulation of inflammatory cytokines(PMID: 12011456). Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA2 protein(PMID: 1463770). This antibody can recognize SAA1 and SAA2.
实验方案
Product Specific Protocols | |
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WB protocol for SAA antibody 13192-1-AP | Download protocol |
IHC protocol for SAA antibody 13192-1-AP | Download protocol |
IF protocol for SAA antibody 13192-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Inflammation Interleukin-1β Induces Intracellular Serum Amyloid A1 Expression in Human Coronary Artery Endothelial Cells and Promotes its Intercellular Exchange. | ||
Int Immunopharmacol Artesunate attenuates serum amyloid A-induced M1 macrophage differentiation through the promotion of PHGDH | ||
Oncol Lett Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma | ||
bioRxiv Single cell RNA sequencing reveals a shift in cell function and maturation of endogenous and infiltrating cell types in response to acute intervertebral disc injury |