验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue, A549 cells, mouse kidney tissue, mouse lung tissue, human brain tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13033-1-AP targets SACM1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SACM1L fusion protein Ag3682 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SAC1 suppressor of actin mutations 1-like (yeast) |
| 别名 | Suppressor of actin mutations 1-like protein, SAC1, Phosphatidylinositol-4-phosphate phosphatase, Phosphatidylinositol-3-phosphatase SAC1, KIAA0851 |
| 计算分子量 | 587 aa, 67 kDa |
| 观测分子量 | 60-67 kDa |
| GenBank蛋白编号 | BC016559 |
| 基因名称 | SACM1L |
| Gene ID (NCBI) | 22908 |
| RRID | AB_2301284 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NTJ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SACM1L(Suppressor of actin mutations 1-like protein) is also named KIAA0851. It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SACM1L antibody 13033-1-AP | Download protocol |
| IHC protocol for SACM1L antibody 13033-1-AP | Download protocol |
| IF protocol for SACM1L antibody 13033-1-AP | Download protocol |
| IP protocol for SACM1L antibody 13033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. | ||
Neuron An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity. | ||
Autophagy PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes.
| ||
Proc Natl Acad Sci U S A Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells. | ||
J Cell Biol Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking. |
