验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, K-562 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68656-1-Ig targets RNF7 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | RNF7 fusion protein Ag16903 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 7 |
| 别名 | CKBBP1, CKII beta binding protein 1, Rbx2, Regulator of cullins 2, RING box protein 2, ring finger protein 7, RNF7, ROC2, SAG |
| 计算分子量 | 113 aa, 13 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC008627 |
| 基因名称 | RNF7 |
| Gene ID (NCBI) | 9616 |
| RRID | AB_3670398 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UBF6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF (ring finger protein 7 ) is a novel zinc RING finger protein which is redox responsive and protects mammalian cells from apoptosis.It is shown to be localised in the cytoplasmand nucleus of the cell and is expressed in the heart, liver, skeletal muscle predominantly. RNF7 forms oligomers(55 kDa),dimer(28 kDa) and monomer(13 kDa) in the presence of different concentrations of the reductant.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF7 antibody 68656-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |