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RNF7 Monoclonal antibody

RNF7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9E7

Cat no : 68656-1-Ig

Print datasheet

Synonyms

CKBBP1, CKII beta binding protein 1, Rbx2, Regulator of cullins 2, RING box protein 2, ring finger protein 7, RNF7, ROC2, SAG

验证数据展示

  • WB analysis using 68656-1-Ig

    WB analysis using 68656-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68656-1-Ig (RNF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, K-562 cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68656-1-Ig targets RNF7 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen RNF7 fusion protein Ag16903 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ring finger protein 7
Synonyms CKBBP1, CKII beta binding protein 1, Rbx2, Regulator of cullins 2, RING box protein 2, ring finger protein 7, RNF7, ROC2, SAG
Calculated Molecular Weight 113 aa, 13 kDa
Observed Molecular Weight13 kDa
GenBank Accession NumberBC008627
Gene Symbol RNF7
Gene ID (NCBI) 9616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UBF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF (ring finger protein 7 ) is a novel zinc RING finger protein which is redox responsive and protects mammalian cells from apoptosis.It is shown to be localised in the cytoplasmand nucleus of the cell and is expressed in the heart, liver, skeletal muscle predominantly. RNF7 forms oligomers(55 kDa),dimer(28 kDa) and monomer(13 kDa) in the presence of different concentrations of the reductant.

实验方案

Product Specific Protocols
WB protocol for RNF7 antibody 68656-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols