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SALL4 Polyclonal antibody
SALL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | mouse testis tissue, human ovary tumor tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Caco-2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24500-1-AP targets SALL4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | SALL4 fusion protein Ag17480 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | sal-like 4 (Drosophila) |
Synonyms | Zinc finger protein SALL4, Zinc finger protein 797, Sal-like protein 4, Sal like protein 4, HSAL4 |
Calculated Molecular Weight | 1053 aa, 112 kDa |
Observed Molecular Weight | 66-75 kDa |
GenBank Accession Number | BC111714 |
Gene Symbol | SALL4 |
Gene ID (NCBI) | 57167 |
RRID | AB_2879576 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UJQ4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SALL4 antibody 24500-1-AP | Download protocol |
IHC protocol for SALL4 antibody 24500-1-AP | Download protocol |
IF protocol for SALL4 antibody 24500-1-AP | Download protocol |
IP protocol for SALL4 antibody 24500-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Rep Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin. | ||
Cell Signal miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis. | ||
Sci Rep MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1. | ||
Pathol Int Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1 | ||
J Mol Histol Expression and clinical significance of SALL4 and β-catenin in colorectal cancer. |