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验证数据展示

  • IF Staining of Caco-2 using 83039-4-RR

    IF Staining of Caco-2 using 83039-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (83039-4-RR, Clone: 230284C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of Caco-2 using 83039-4-RR

    IF Staining of Caco-2 using 83039-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (83039-4-RR, Clone: 230284C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83039-4-RR

    Affinity and Kinetic Characterization of 83039-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83039-4-RR against Human SALL4 were performed. The affinity constant is 0.18 nM.

SALL4 Recombinant antibody

SALL4 Recombinant Antibody for IF/ICC, ELISA
Cat No. 83039-4-RR

产品说明书

CloneNo. 230284C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

Zinc finger protein 797, Sal-like protein 4, Sal like protein 4, HSAL4, DRRS

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83039-4-RR targets SALL4 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SALL4 fusion protein Ag16076 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sal-like 4 (Drosophila)
别名 Zinc finger protein 797, Sal-like protein 4, Sal like protein 4, HSAL4, DRRS
计算分子量 1053 aa, 112 kDa
GenBank蛋白编号BC111714
基因名称 SALL4
Gene ID (NCBI) 57167
RRIDAB_3670770
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UJQ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SALL4, also named Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4 in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.

实验方案

Product Specific Protocols
IF protocol for SALL4 antibody 83039-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols