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  • Featured Product
  • KD/KO Validated

SAMD4B Polyclonal antibody

SAMD4B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17723-1-AP

Print datasheet

Synonyms

hSmaug2, Protein Smaug homolog 2, SAMD4B, Smaug 2, SMAUG2, SMGB

验证数据展示

  • WB analysis of HeLa using 17723-1-AP

    WB analysis of HeLa using 17723-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17723-1-AP

    IF Staining of HepG2 using 17723-1-AP

    Immunofluorescent analysis of HepG2 cells, using SAMD4B antibody 17723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SAMD4B Ready-To-Use IHC Kit. SAMD4B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17723-1-AP targets SAMD4B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SAMD4B fusion protein Ag12113 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterile alpha motif domain containing 4B
Synonyms hSmaug2, Protein Smaug homolog 2, SAMD4B, Smaug 2, SMAUG2, SMGB
Calculated Molecular Weight 694 aa, 75 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC054518
Gene Symbol SAMD4B
Gene ID (NCBI) 55095
RRIDAB_2183381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5PRF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAMD4B antibody 17723-1-APDownload protocol
IF protocol for SAMD4B antibody 17723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Immunol

SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.

Authors - Yuze Wang
  • KO Validated
View Article
humanWB,IF

Mol Med Rep

miR‑451 suppresses the malignant characteristics of colorectal cancer via targeting SAMD4B.

Authors - Chunrong Wu
View Article