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SAMD8 Polyclonal antibody

SAMD8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26815-1-AP

Print datasheet

Synonyms

SAMD8, SMSr

验证数据展示

  • WB analysis of mouse heart using 26815-1-AP

    WB analysis of mouse heart using 26815-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 26815-1-AP (SAMD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26815-1-AP

    IHC staining of human liver cancer using 26815-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 26815-1-AP

    IHC staining of human liver cancer using 26815-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26815-1-AP targets SAMD8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen SAMD8 fusion protein Ag25310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterile alpha motif domain containing 8
Synonyms SAMD8, SMSr
Observed Molecular Weight 38 kDa, 45 kDa
GenBank Accession NumberBC146556
Gene Symbol SAMD8
Gene ID (NCBI) 142891
RRIDAB_2880644
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LT4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAMD8 antibody 26815-1-APDownload protocol
IHC protocol for SAMD8 antibody 26815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

J Biol Chem

Sphingomyelin synthase-related protein SMSr is a phosphatidylethanolamine phospholipase C that promotes nonalcoholic fatty liver disease

Authors - Yeun-Po Chiang
View Article