• Featured Product
  • KD/KO Validated

Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20824-1-AP

Print datasheet

Synonyms

SAMM50, TOB55, SAMM 50, OMP85, CGI-51



经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, A375 cells, mouse ovary tissue, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20824-1-AP targets Sam50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen Sam50 fusion protein Ag14799 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Synonyms SAMM50, TOB55, SAMM 50, OMP85, CGI-51
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007830
Gene Symbol Sam50
Gene ID (NCBI) 25813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

实验方案

Product Specific Protocols
WB protocol for Sam50 antibody 20824-1-APDownload protocol
IHC protocol for Sam50 antibody 20824-1-APDownload protocol
IF protocol for Sam50 antibody 20824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

Authors - Virginia Guarani
humanWB

Cell Rep

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

Authors - Timothy D Martin
  • KD Validated
mouseWB

Aging Cell

3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex

Authors - Zer Vue
ratWB

Free Radic Biol Med

Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy.

Authors - Run-Qing Xue
humanWB

J Cell Biol

The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy

Authors - Mehmet Oguz Gok
pigIF

Elife

Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system

Authors - Dalen Zuidema