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Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20824-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

SAMM50, TOB55, SAMM 50, OMP85, CGI-51

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, A375 cells, mouse ovary tissue, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20824-1-AP targets Sam50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 Sam50 fusion protein Ag14799 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting and assembly machinery component 50 homolog (S. cerevisiae)
别名 SAMM50, TOB55, SAMM 50, OMP85, CGI-51
计算分子量 469 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC007830
基因名称 Sam50
Gene ID (NCBI) 25813
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y512
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

实验方案

Product Specific Protocols
WB protocol for Sam50 antibody 20824-1-APDownload protocol
IHC protocol for Sam50 antibody 20824-1-APDownload protocol
IF protocol for Sam50 antibody 20824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

Authors - Virginia Guarani
humanWB

Cell Rep

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

Authors - Timothy D Martin
  • KD Validated
mouseWB

Aging Cell

3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex

Authors - Zer Vue
ratWB

Free Radic Biol Med

Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy.

Authors - Run-Qing Xue
humanWB

J Cell Biol

The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy

Authors - Mehmet Oguz Gok
pigIF

Elife

Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system

Authors - Dalen Zuidema
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