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Sam50 Polyclonal antibody
Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, A431 cells, A375 cells, mouse ovary tissue, mouse kidney tissue |
Positive IHC detected in | human hepatocirrhosis tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20824-1-AP targets Sam50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | Sam50 fusion protein Ag14799 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | sorting and assembly machinery component 50 homolog (S. cerevisiae) |
Synonyms | SAMM50, TOB55, SAMM 50, OMP85, CGI-51 |
Calculated Molecular Weight | 469 aa, 52 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC007830 |
Gene Symbol | Sam50 |
Gene ID (NCBI) | 25813 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y512 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Sam50 antibody 20824-1-AP | Download protocol |
IHC protocol for Sam50 antibody 20824-1-AP | Download protocol |
IF protocol for Sam50 antibody 20824-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Elife QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. | ||
Cell Rep A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.
| ||
Aging Cell 3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex | ||
Free Radic Biol Med Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy. | ||
J Cell Biol The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy | ||
Elife Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system |