验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SAMSN1 Ready-To-Use IHC Kit. SAMSN1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
13063-1-AP targets SAMSN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SAMSN1 fusion protein Ag3755 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SAM domain, SH3 domain and nuclear localization signals 1 |
| 别名 | SH3-SAM adaptor protein, SH3D6B, SH3 SAM adaptor protein, SAM domain-containing protein SAMSN-1, HACS1 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42-50 kDa |
| GenBank蛋白编号 | BC029112 |
| 基因名称 | SAMSN1 |
| Gene ID (NCBI) | 64092 |
| RRID | AB_2877910 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NSI8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAMSN1 antibody 13063-1-AP | Download protocol |
| IHC protocol for SAMSN1 antibody 13063-1-AP | Download protocol |
| IF protocol for SAMSN1 antibody 13063-1-AP | Download protocol |
| IP protocol for SAMSN1 antibody 13063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cells Int Lama2 And Samsn1 Mediate the Effects of Brn4 on Hippocampal Neural Stem Cell Proliferation and Differentiation
| ||
Ann Surg Oncol Suppression of SAMSN1 Expression is Associated with the Malignant Phenotype of Hepatocellular Carcinoma. | ||
Mol Cell Proteomics Multi-Level Proteomics Reveals Epigenetic Signatures in BCG-mediated Macrophage Activation |
