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SAP 97 Polyclonal antibody

SAP 97 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29292-1-AP

Print datasheet

Synonyms

Disks large homolog 1, dJ1061C18.1.1, DKFZp761P0818, DKFZp781B0426, DLG1, DLGH1, hdlg, SAP 97, SAP97, Synapse associated protein 97

验证数据展示

  • WB analysis using 29292-1-AP

    WB analysis using 29292-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29292-1-AP (DLG1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29292-1-AP

    IHC staining of human colon cancer using 29292-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLG1 antibody included
  • IF Staining of HEK-293 using 29292-1-AP

    IF Staining of HEK-293 using 29292-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SAP 97 antibody (29292-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HUVEC cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy DLG1 Ready-To-Use IHC Kit. DLG1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29292-1-AP targets SAP 97 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen SAP 97 fusion protein Ag31066 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name discs, large homolog 1 (Drosophila)
Synonyms Disks large homolog 1, dJ1061C18.1.1, DKFZp761P0818, DKFZp781B0426, DLG1, DLGH1, hdlg, SAP 97, SAP97, Synapse associated protein 97
Calculated Molecular Weight 100 kDa
Observed Molecular Weight100-130 kDa
GenBank Accession NumberNM_001098424
Gene Symbol SAP97
Gene ID (NCBI) 1739
RRIDAB_2918273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12959
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DLG1, also naemd as SAP97, hDlg and DLGH1, belongs to the MAGUK family. It is essential multidomain scaffolding protein required for normal development. DLG1 may play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. It regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. DLG1 is a functional regulator of Kv1.5 channel. The DLG1-MPP7 complex promotes epithelial cell polarity and tight junction formation. The antibody is specific to DLG1.

实验方案

Product Specific Protocols
WB protocol for SAP 97 antibody 29292-1-APDownload protocol
IHC protocol for SAP 97 antibody 29292-1-APDownload protocol
IF protocol for SAP 97 antibody 29292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols