验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HL-60 cells, K-562 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
27679-1-AP targets SAP30 in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | SAP30 fusion protein Ag26516 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Sin3A-associated protein, 30kDa |
| Synonyms | SAP30 |
| Calculated Molecular Weight | 220 aa, 23 kDa |
| Observed Molecular Weight | 23-28 kDa |
| GenBank Accession Number | BC016757 |
| Gene Symbol | SAP30 |
| Gene ID (NCBI) | 8819 |
| RRID | AB_2880945 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75446 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAP30, also named as Sin3 corepressor complex subunit SAP30, is a 220 amino acid protein, which is expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAP30 antibody 27679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Development Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures. | ||
Cell Rep SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns |
