验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HL-60 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
27679-1-AP targets SAP30 in WB, IF, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | SAP30 fusion protein Ag26516 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Sin3A-associated protein, 30kDa |
| 别名 | SAP30 |
| 计算分子量 | 220 aa, 23 kDa |
| 观测分子量 | 23-28 kDa |
| GenBank蛋白编号 | BC016757 |
| 基因名称 | SAP30 |
| Gene ID (NCBI) | 8819 |
| RRID | AB_2880945 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75446 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAP30, also named as Sin3 corepressor complex subunit SAP30, is a 220 amino acid protein, which is expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAP30 antibody 27679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Development Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures. | ||
Cell Rep SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns |
