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验证数据展示

  • WB analysis of HeLa using 17819-1-AP

    WB analysis of HeLa using 17819-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17819-1-AP (SAPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17819-1-AP

    IHC staining of human testis using 17819-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17819-1-AP (SAPS1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 17819-1-AP

    IHC staining of human testis using 17819-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17819-1-AP (SAPS1 antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of HeLa using 17819-1-AP

    IP experiment of HeLa using 17819-1-AP

    IP result of anti-SAPS1 (IP:17819-1-AP, 4ug; Detection:17819-1-AP 1:1000) with HeLa cells lysate 3200ug.

SAPS1 Polyclonal antibody

SAPS1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17819-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

KIAA1115, PP6R1, PPP6R1, SAP190, SAPS domain family member 1, SAPS domain family, member 1, SAPS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 1 publications below

产品信息

17819-1-AP targets SAPS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IP
经测试反应性 human, mouse
文献引用反应性human
免疫原 SAPS1 fusion protein Ag12149 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SAPS domain family, member 1
别名 KIAA1115, PP6R1, PPP6R1, SAP190, SAPS domain family member 1, SAPS domain family, member 1, SAPS1
计算分子量881aa,97 kDa; 943aa,103 kDa
观测分子量 120 kDa
GenBank蛋白编号BC094753
基因名称 SAPS1
Gene ID (NCBI) 22870
RRIDAB_2183809
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPN7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAPS1 antibody 17819-1-APDownload protocol
IHC protocol for SAPS1 antibody 17819-1-APDownload protocol
IP protocol for SAPS1 antibody 17819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Cell Death Dis

PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death

Authors - Guowei Wu
View Article