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SAPS1 Polyclonal antibody

SAPS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17819-1-AP

Print datasheet

Synonyms

KIAA1115, PP6R1, PPP6R1, SAP190, SAPS domain family member 1, SAPS domain family, member 1, SAPS1

验证数据展示

  • WB analysis of HeLa using 17819-1-AP

    WB analysis of HeLa using 17819-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17819-1-AP (SAPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17819-1-AP

    IHC staining of human testis using 17819-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17819-1-AP (SAPS1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 17819-1-AP

    IHC staining of human testis using 17819-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17819-1-AP (SAPS1 antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of HeLa using 17819-1-AP

    IP experiment of HeLa using 17819-1-AP

    IP result of anti-SAPS1 (IP:17819-1-AP, 4ug; Detection:17819-1-AP 1:1000) with HeLa cells lysate 3200ug.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 1 publications below

产品信息

17819-1-AP targets SAPS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SAPS1 fusion protein Ag12149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SAPS domain family, member 1
Synonyms KIAA1115, PP6R1, PPP6R1, SAP190, SAPS domain family member 1, SAPS domain family, member 1, SAPS1
Calculated Molecular Weight881aa,97 kDa; 943aa,103 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC094753
Gene Symbol SAPS1
Gene ID (NCBI) 22870
RRIDAB_2183809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPN7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAPS1 antibody 17819-1-APDownload protocol
IHC protocol for SAPS1 antibody 17819-1-APDownload protocol
IP protocol for SAPS1 antibody 17819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Cell Death Dis

PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death

Authors - Guowei Wu
View Article