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验证数据展示

  • WB analysis using 26190-1-AP

    WB analysis using 26190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26190-1-AP (SAPS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 26190-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26190-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26190-1-AP (SAPS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26190-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26190-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26190-1-AP (SAPS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 26190-1-AP

    WB analysis of HepG2 using 26190-1-AP

    WB result of SAPS2 antibody (26190-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAPS2 transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

SAPS2 Polyclonal antibody

SAPS2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26190-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Serine/threonine-protein phosphatase 6 regulatory subunit 2, Serine/threonine protein phosphatase 6 regulatory subunit 2, SAPS domain family, member 2, SAPS domain family member 2, SAPS 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26190-1-AP targets SAPS2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 SAPS2 fusion protein Ag23458 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SAPS domain family, member 2
别名 Serine/threonine-protein phosphatase 6 regulatory subunit 2, Serine/threonine protein phosphatase 6 regulatory subunit 2, SAPS domain family, member 2, SAPS domain family member 2, SAPS 2
计算分子量 105 kDa
观测分子量115-125 kDa
GenBank蛋白编号BC000976
基因名称 SAPS2
Gene ID (NCBI) 9701
RRIDAB_3085847
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75170
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAPS2 is a regulatory protein for the protein phosphatase-6 catalytic subunit. Together, these proteins act as a significant T-loop phosphatase for Aurora A, an essential mitotic kinase. Loss of function of either the regulatory or catalytic subunit of protein phosphatase-6 interferes with spindle formation and chromosome alignment.

实验方案

Product Specific Protocols
WB protocol for SAPS2 antibody 26190-1-APDownload protocol
IHC protocol for SAPS2 antibody 26190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols