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验证数据展示

  • WB analysis using 15453-1-AP

    WB analysis using 15453-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15453-1-AP (SAR1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 15453-1-AP

    IHC staining of human stomach cancer using 15453-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15453-1-AP (SAR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 15453-1-AP

    IHC staining of human stomach cancer using 15453-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15453-1-AP (SAR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SAR1B Polyclonal antibody

SAR1B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15453-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, mouse brain tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15453-1-AP targets SAR1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SAR1B fusion protein Ag7718 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SAR1 homolog B (S. cerevisiae)
别名 ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB
计算分子量 22 kDa
观测分子量 25-27 kDa
GenBank蛋白编号BC002847
基因名称 SAR1B
Gene ID (NCBI) 51128
RRIDAB_2183985
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6B6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex.

实验方案

Product Specific Protocols
WB protocol for SAR1B antibody 15453-1-APDownload protocol
IHC protocol for SAR1B antibody 15453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols