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SARS-CoV-2 S protein (319-541 aa) Polyclonal antibody

SARS-CoV-2 S protein (319-541 aa) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Virus

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28991-1-AP

Print datasheet

Synonyms

SARS-CoV-2 Spike Glycoprotein (319-541 aa)

验证数据展示

  • WB analysis using 28991-1-AP

    WB analysis using 28991-1-AP

    E.coli expressed SARS-CoV-2 spike protein (Cat.NO. Ag30688) was subjected to SDS PAGE followed by western blot with 28991-1-AP (SARS-CoV-2 S protein (319-541 aa) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • ELISA experiment of Recombinant protein using 28991-1-AP

    ELISA experiment of Recombinant protein using 28991-1-AP

    SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP) tested by ELISA. SARS-CoV-2 Spike Glycoprotein (319-541 aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

经过测试的应用

Positive WB detected inRecombinant protein
Positive ELISA detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28991-1-AP targets SARS-CoV-2 S protein (319-541 aa) in WB, ELISA applications and shows reactivity with Virus samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Virus
Immunogen SARS-CoV-2 S protein (319-541 aa) fusion protein Ag30689 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SARS-CoV-2 Spike Protein
Synonyms SARS-CoV-2 Spike Glycoprotein (319-541 aa)
Calculated Molecular Weight 141 kDa
GenBank Accession NumberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28991-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 319-541aa.

实验方案

Product Specific Protocols
WB protocol for SARS-CoV-2 S protein (319-541 aa) antibody 28991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols