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验证数据展示

  • WB analysis using 28991-1-AP

    WB analysis using 28991-1-AP

    E.coli expressed SARS-CoV-2 spike protein (Cat.NO. Ag30688) was subjected to SDS PAGE followed by western blot with 28991-1-AP (SARS-CoV-2 S protein (319-541 aa) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • ELISA experiment of Recombinant protein using 28991-1-AP

    ELISA experiment of Recombinant protein using 28991-1-AP

    SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP) tested by ELISA. SARS-CoV-2 Spike Glycoprotein (319-541 aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

SARS-CoV-2 S protein (319-541 aa) Polyclonal antibody

SARS-CoV-2 S protein (319-541 aa) Polyclonal Antibody for WB, ELISA
Cat No. 28991-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Virus

应用

WB, ELISA

别名

SARS-CoV-2 Spike Glycoprotein (319-541 aa)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein
Positive ELISA detected inRecombinant protein

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28991-1-AP targets SARS-CoV-2 S protein (319-541 aa) in WB, ELISA applications and shows reactivity with Virus samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Virus
免疫原 SARS-CoV-2 S protein (319-541 aa) fusion protein Ag30689 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SARS-CoV-2 Spike Protein
别名 SARS-CoV-2 Spike Glycoprotein (319-541 aa)
计算分子量 141 kDa
GenBank蛋白编号NC_045512
基因名称 SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_3086093
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28991-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 319-541aa.

实验方案

Product Specific Protocols
WB protocol for SARS-CoV-2 S protein (319-541 aa) antibody 28991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols