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SARS-CoV-2 S protein (944-1214 aa) Monoclonal antibody

SARS-CoV-2 S protein (944-1214 aa) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Virus

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H5E6

Cat no : 67794-1-Ig

Print datasheet

Synonyms

S protein, SARS-CoV-2 Spike Glycoprotein, SARS-CoV-2 Spike Glycoprotein (944-1214 aa), spike, spike protein

验证数据展示

  • WB analysis of Recombinant protein using 67794-1-Ig

    WB analysis of Recombinant protein using 67794-1-Ig

    Spike Glycoprotein (944-1214 aa) were subjected to SDS PAGE followed by western blot with 67794-1-lg (SARS-CoV-2 Spike Glycoprotein (944-1214 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inag30682

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67794-1-Ig targets SARS-CoV-2 S protein (944-1214 aa) in WB, ELISA applications and shows reactivity with Virus samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Virus
Immunogen SARS-CoV-2 S protein (944-1214 aa) fusion protein Ag30682 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SARS-CoV-2 Spike Protein
Synonyms S protein, SARS-CoV-2 Spike Glycoprotein, SARS-CoV-2 Spike Glycoprotein (944-1214 aa), spike, spike protein
Calculated Molecular Weight 141 kDa
GenBank Accession NumberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_2918558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).

实验方案

Product Specific Protocols
WB protocol for SARS-CoV-2 S protein (944-1214 aa) antibody 67794-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols