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SARS2 Polyclonal antibody

SARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17258-1-AP

Print datasheet

Synonyms

Serine--tRNA ligase, mitochondrial, Serine  tRNA ligase, SARSM, SARS, mtSerRS

验证数据展示

  • IF Staining of HepG2 using 17258-1-AP

    IF Staining of HepG2 using 17258-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17258-1-AP (SARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 17258-1-AP

    WB analysis using 17258-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 17258-1-AP

    IHC staining of human lung cancer using 17258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 17258-1-AP

    IHC staining of human lung cancer using 17258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse liver tissue, HepG2 cells, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17258-1-AP targets SARS2 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SARS2 fusion protein Ag10913 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name seryl-tRNA synthetase 2, mitochondrial
Synonyms Serine--tRNA ligase, mitochondrial, Serine  tRNA ligase, SARSM, SARS, mtSerRS
Calculated Molecular Weight 518 aa, 58 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC042912
Gene Symbol SARS2
Gene ID (NCBI) 54938
RRIDAB_2238863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SARS2 antibody 17258-1-APDownload protocol
IHC protocol for SARS2 antibody 17258-1-APDownload protocol
IF protocol for SARS2 antibody 17258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.

Authors - Shasha Gong
View Article
humanWB,RIP

Oncogene

NSUN2 regulates Wnt signaling pathway depending on the m5C RNA modification to promote the progression of hepatocellular carcinoma

Authors - Huiwu Xing
View Article