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验证数据展示

  • WB analysis of HeLa using 22675-1-AP (same clone as 22675-1-PBS)

    WB analysis of HeLa using 22675-1-AP (same clone as 22675-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22675-1-AP (SART1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 22675-1-AP (same clone as 22675-1-PBS)

    IP experiment of mouse brain using 22675-1-AP (same clone as 22675-1-PBS)

    IP result of anti-SART1 (IP:22675-1-AP, 4ug; Detection:22675-1-AP 1:6000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 22675-1-AP (same clone as 22675-1-PBS)

    IHC staining of mouse testis using 22675-1-AP (same clone as 22675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 22675-1-AP (same clone as 22675-1-PBS)

    IHC staining of mouse testis using 22675-1-AP (same clone as 22675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 22675-1-AP (same clone as 22675-1-PBS)

    IF Staining of HepG2 using 22675-1-AP (same clone as 22675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SART1 antibody (22675-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SART1 Polyclonal antibody, PBS Only

SART1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22675-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

hSnu66, hSART-1, hSART 1, HOMS1, Hom s 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

22675-1-PBS targets SART1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SART1 fusion protein Ag18478 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 squamous cell carcinoma antigen recognized by T cells
别名 hSnu66, hSART-1, hSART 1, HOMS1, Hom s 1
计算分子量 800 aa, 90 kDa
观测分子量 110 kDa
GenBank蛋白编号BC001058
基因名称 SART1
Gene ID (NCBI) 9092
RRIDAB_2879148
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43290
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Squamous cell carcinoma antigen recognized by T cells (SART1), also known as U4/U6.U5 tri-snRNP-associated 110 kDa protein, is a ubiquitously expressed protein that is involved in mRNA splicing and the regulation of cell proliferation. It has been identified as regulator of c-FLIP and drug-induced activation of caspase 8. The SART1 gene encodes two proteins, the SART1(800) protein is located in the nucleus of the majority of proliferating cells, while the SART1(259) protein is located in the cytosol of epithelial cancers. Genetic variation in the SART1 gene may be associated with breast cancer development. This antibody recognizes the endogenous 800-amino acid SART1, which migrates with an apparent molecular mass of 110 kDa in SDS-PAGE. (PMID: 22027693; 9449708; 19377877; 11350945)