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验证数据展示

  • WB analysis of Jurkat using 18025-1-AP

    WB analysis of Jurkat using 18025-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 18025-1-AP

    IP experiment of HeLa using 18025-1-AP

    IP result of anti-SART3 (IP:18025-1-AP, 4ug; Detection:18025-1-AP 1:700) with HeLa cells lysate 2000ug.

  • IHC staining of human lung cancer using 18025-1-AP

    IHC staining of human lung cancer using 18025-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SART3 antibody included
  • IHC staining of mouse kidney using 18025-1-AP

    IHC staining of mouse kidney using 18025-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SART3 antibody included
  • IHC staining of rat kidney using 18025-1-AP

    IHC staining of rat kidney using 18025-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SART3 antibody included
  • IHC staining of human stomach cancer using 18025-1-AP

    IHC staining of human stomach cancer using 18025-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SART3 antibody included

SART3 Polyclonal antibody

SART3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18025-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

p110 nuclear RNA-binding protein, p110, P100, KIAA0156, hSART 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human lung cancer tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SART3 Ready-To-Use IHC Kit. SART3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18025-1-AP targets SART3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SART3 fusion protein Ag12590 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 squamous cell carcinoma antigen recognized by T cells 3
别名 p110 nuclear RNA-binding protein, p110, P100, KIAA0156, hSART 3
计算分子量 963 aa, 110 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC093784
基因名称 SART3
Gene ID (NCBI) 9733
RRIDAB_2254228
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15020
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The process of mRNA splicing required assembly of pre-mRNA into a large, dynamic RNA-protein complex, in which 5 small nuclear RNAs and more than 50 protein components are incorporated into functional small nuclear ribonucleoproteins(snRNPs). SART3 interacts with U6 snRNA and functioned as a U6-specific recycling factor responsible for regeneration of base-paired U4/U6 snRNPs from post-spliceosomal free U4 and U6 snRNPs. Also it can interact and regulate Tat transactivation activity, and act as a cellular factor for HIV-1 gene expression and viral replication.

实验方案

Product Specific Protocols
WB protocol for SART3 antibody 18025-1-APDownload protocol
IHC protocol for SART3 antibody 18025-1-APDownload protocol
IP protocol for SART3 antibody 18025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIP

RNA

7SL RNA in Vertebrate Red Blood Cells.

Authors - Gaëlle J S Talhouarne
View Article
humanWB,IP

J Biol Chem

Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.

Authors - Zhe Chen
View Article

Mol Cell Biol

USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis.

Authors - Jaehyun Kim
View Article
humanIHC

Aging (Albany NY)

SART3, regulated by p53, is a biomarker for diagnosis, prognosis and immune infiltration in hepatocellular carcinoma

Authors - Jusen Nong
View Article