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SASH1 Polyclonal antibody

SASH1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27689-1-AP

Print datasheet

Synonyms

SH3D6A, SAM and SH3 domain-containing protein 1, RP3 323M4.1, Proline-glutamate repeat-containing protein, PEPE1

验证数据展示

  • WB analysis using 27689-1-AP

    WB analysis using 27689-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27689-1-AP (SASH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSKOV-3 cells, U-251 cells, U-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27689-1-AP targets SASH1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen SASH1 fusion protein Ag26771 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SAM and SH3 domain containing 1
Synonyms SH3D6A, SAM and SH3 domain-containing protein 1, RP3 323M4.1, Proline-glutamate repeat-containing protein, PEPE1
Calculated Molecular Weight 137 kDa
Observed Molecular Weight170 kDa
GenBank Accession NumberNM_015278
Gene Symbol SASH1
Gene ID (NCBI) 23328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAM and SH3 domain-containing protein 1 (SASH1, also known as KIAA0790 and PEPE1) is a multidomain protein implicated in various cellular processes, including cell adhesion, migration, and apoptosis. It has been identified as a novel Eph receptor-binding partner through SAM-SAM domain interactions, which are critical for the recruitment and binding of downstream molecules in the Eph signaling pathway (PMID: 37619706). SASH1 is also known to regulate signaling through focal adhesion kinase (FAK) and AKT/PI3K and promote apoptosis. Mutations in SASH1 have been associated with cancers, and it is suggested as a potential therapeutic target in non-small cell lung cancer (PMID: 33122723).

实验方案

Product Specific Protocols
WB protocol for SASH1 antibody 27689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols