验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human testis tissue, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21377-1-AP targets SASS6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | SASS6 fusion protein Ag15917 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spindle assembly 6 homolog (C. elegans) |
| 别名 | HsSAS 6, SAS 6, SAS6, SASS6 |
| 计算分子量 | 657 aa, 74 kDa |
| 观测分子量 | 70-74 kDa |
| GenBank蛋白编号 | BC101026 |
| 基因名称 | SASS6 |
| Gene ID (NCBI) | 163786 |
| RRID | AB_2878851 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UVJ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SASS6 antibody 21377-1-AP | Download protocol |
| IHC protocol for SASS6 antibody 21377-1-AP | Download protocol |
| IP protocol for SASS6 antibody 21377-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells. | ||
Oncol Rep Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase. | ||
EBioMedicine Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities | ||
Clin Exp Med SASS6 promotes tumor proliferation and is associated with TP53 and immune infiltration in lung adenocarcinoma |
