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验证数据展示

  • WB analysis using 19557-1-AP

    WB analysis using 19557-1-AP

    The lysate of total HeLa cells and nuclear extracted from HeLa cells were subjected to SDS PAGE followed by western blot with 19557-1-AP (SATB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 19557-1-AP

    IHC staining of human colon cancer using 19557-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 19557-1-AP (SATB2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SATB2 Polyclonal antibody

SATB2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19557-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SATB homeobox 2, KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19557-1-AP targets SATB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SATB homeobox 2
别名 SATB homeobox 2, KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2
计算分子量 83 kDa
观测分子量~100 kDa
GenBank蛋白编号NM_015265
基因名称 SATB2
Gene ID (NCBI) 23314
RRIDAB_3085581
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPW6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

实验方案

Product Specific Protocols
WB protocol for SATB2 antibody 19557-1-APDownload protocol
IHC protocol for SATB2 antibody 19557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols