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验证数据展示

  • WB analysis using 19557-1-AP

    WB analysis using 19557-1-AP

    The lysate of total HeLa cells and nuclear extracted from HeLa cells were subjected to SDS PAGE followed by western blot with 19557-1-AP (SATB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 19557-1-AP

    IHC staining of human colon cancer using 19557-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 19557-1-AP (SATB2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SATB2 Polyclonal antibody

SATB2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19557-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SATB homeobox 2, KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19557-1-AP targets SATB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SATB homeobox 2
Synonyms SATB homeobox 2, KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2
Calculated Molecular Weight 83 kDa
Observed Molecular Weight~100 kDa
GenBank Accession NumberNM_015265
Gene Symbol SATB2
Gene ID (NCBI) 23314
RRIDAB_3085581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPW6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

实验方案

Product Specific Protocols
WB protocol for SATB2 antibody 19557-1-APDownload protocol
IHC protocol for SATB2 antibody 19557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols