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验证数据展示

  • WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67958-1-Ig (same clone as 67958-1-PBS)

    WB analysis of HEK-293 using 67958-1-Ig (same clone as 67958-1-PBS)

    WB result of SATB2 antibody (67958-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SATB2 transfected HEK-293 cells. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    HepG2 cell lysates and HepG2 nuclear protein were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67958-1-Ig (same clone as 67958-1-PBS)

    IHC staining of human colon cancer using 67958-1-Ig (same clone as 67958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50682-1

    Cytometric bead array standard curve of MP50682-1

    Cytometric bead array standard curve of MP50682-1, SATB2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67958-1-PBS. Detection antibody: 67958-2-PBS. Standard:Ag17237. Range: 0.781-100 ng/mL.

  • IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SATB2 Monoclonal antibody, PBS Only (Capture)

SATB2 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67958-1-PBS

产品说明书

CloneNo. 2F8E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2, DNA binding protein SATB2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67958-1-PBS targets SATB2 as part of a matched antibody pair:

MP50682-1: 67958-1-PBS capture and 67958-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SATB2 fusion protein Ag17237 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SATB homeobox 2
别名 KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2, DNA binding protein SATB2
计算分子量 733 aa, 83 kDa
观测分子量85-100 kDa
GenBank蛋白编号BC098136
基因名称 SATB2
Gene ID (NCBI) 23314
RRIDAB_2918709
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UPW6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. SATB2 has two isoforms produced by alternative splicing with the MW of 70 kDa and 83 kDa. It can be detected as 85-105 kDa by sumo modification (PMID: 14701874, PMID: 35140581).