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  • Featured Product
  • KD/KO Validated

SATB2 Monoclonal antibody, PBS Only (Capture)

SATB2 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F8E2

Cat no : 67958-1-PBS

Print datasheet

Synonyms

KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2, DNA binding protein SATB2

验证数据展示

  • WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67958-1-Ig (same clone as 67958-1-PBS)

    WB analysis of HEK-293 using 67958-1-Ig (same clone as 67958-1-PBS)

    WB result of SATB2 antibody (67958-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SATB2 transfected HEK-293 cells. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    HepG2 cell lysates and HepG2 nuclear protein were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50682-1

    Cytometric bead array standard curve of MP50682-1

    Cytometric bead array standard curve of MP50682-1, SATB2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67958-1-PBS. Detection antibody: 67958-2-PBS. Standard:Ag17237. Range: 0.781-100 ng/mL.

  • IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

产品信息

67958-1-PBS targets SATB2 as part of a matched antibody pair:

MP50682-1: 67958-1-PBS capture and 67958-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen SATB2 fusion protein Ag17237 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SATB homeobox 2
Synonyms KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2, DNA binding protein SATB2
Calculated Molecular Weight 733 aa, 83 kDa
Observed Molecular Weight85-100 kDa
GenBank Accession NumberBC098136
Gene Symbol SATB2
Gene ID (NCBI) 23314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UPW6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. SATB2 has two isoforms produced by alternative splicing with the MW of 70 kDa and 83 kDa. It can be detected as 85-105 kDa by sumo modification (PMID: 14701874, PMID: 35140581).