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验证数据展示

  • WB analysis using 25359-1-AP

    WB analysis using 25359-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25359-1-AP

    IF Staining of HeLa using 25359-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SBNO1 antibody (25359-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IP experiment of Jurkat using 25359-1-AP

    IP experiment of Jurkat using 25359-1-AP

    IP result of anti-SBNO1 (IP:25359-1-AP, 4ug; Detection:25359-1-AP 1:3000) with Jurkat cells lysate 1400 ug.

SBNO1 Polyclonal antibody

SBNO1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 25359-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

Monocyte protein 3, MOP 3, MOP3, SBNO1, Sno

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25359-1-AP targets SBNO1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 SBNO1 fusion protein Ag19005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 strawberry notch homolog 1 (Drosophila)
别名 Monocyte protein 3, MOP 3, MOP3, SBNO1, Sno
计算分子量 1393 aa, 154 kDa
观测分子量 145-154 kDa
GenBank蛋白编号BC133704
基因名称 SBNO1
Gene ID (NCBI) 55206
RRIDAB_2880042
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA3KN83
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SBNO1, also named as Monocyte protein 3 or MOP3, is a 1393 amino acid protein, which belongs to the SBNO family. SBNO1 play an important role in development of the vertebrate central nervous system. SBNO1 has four isoforms with MW 154 kDa and 80 kDa.

实验方案

Product Specific Protocols
WB protocol for SBNO1 antibody 25359-1-APDownload protocol
IF protocol for SBNO1 antibody 25359-1-APDownload protocol
IP protocol for SBNO1 antibody 25359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Asian J Androl

Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice.

Authors - Cong Shen
View Article