验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | mouse bladder tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15288-1-AP targets SC65 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SC65 fusion protein Ag7480 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | synaptonemal complex protein SC65 |
| 别名 | NOL55, Nucleolar autoantigen No55, P3H4, SC65 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC001047 |
| 基因名称 | SC65 |
| Gene ID (NCBI) | 10609 |
| RRID | AB_2184611 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92791 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SC65, also named as Leprecan-like protein 4 or Nucleolar autoantigen No55, is a 437 amino acid protein, which belongs to the leprecan family. SC65 ia a protein of the interphase nucleolus and tumour-associated autoantigen in patients with prostate cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SC65 antibody 15288-1-AP | Download protocol |
| IP protocol for SC65 antibody 15288-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.
| ||
J Bone Miner Res Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
| ||
Cancers (Basel) P3H4 Promotes Malignant Progression of Lung Adenocarcinoma via Interaction with EGFR.
| ||
PLoS Genet Cyclophilin B control of lysine post-translational modifications of skin type I collagen. | ||
Aging (Albany NY) Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer.
| ||
Sci Rep Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure |
