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验证数据展示

  • WB analysis using 28174-1-AP

    WB analysis using 28174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28174-1-AP (SCARF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SCARF1 Polyclonal antibody

SCARF1 Polyclonal Antibody for WB, ELISA
Cat No. 28174-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

Acetyl LDL receptor, KIAA0149, SCARF1, SREC, SREC-I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, SMMC-7721 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28174-1-AP targets SCARF1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 SCARF1 fusion protein Ag28079 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 scavenger receptor class F, member 1
别名 Acetyl LDL receptor, KIAA0149, SCARF1, SREC, SREC-I
计算分子量 830 aa, 87 kDa
观测分子量90 kDa, 180 kDa
GenBank蛋白编号BC039735
基因名称 SCARF1
Gene ID (NCBI) 8578
RRIDAB_3086035
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14162
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Scavenger receptor class F member 1 (SCARF1), also known as scavenger receptor expressed by endothelial cells (SREC)-I, belongs to the scavenger receptor (SR) superfamily. SCARF1 has been shown to bind modified low density lipoproteins (LDLs), including acetylated (Ac-) and oxidized (ox-) LDLs (PMID: 29725698; PMID: 15145948). In addition to binding and internalizing a diverse range of endogenous proteins, SCARF1 also binds a wide array of viral, fungal, and bacterial antigens (PMID: 29242513). SCARF1 has a calculated molecular weight of 87 kDa, with a molecular weight of 140-160 kDa due to N-glycosylation (PMID: 22279061; PMID: 15145948). SCARF1 can also exist as a dimer (~ 180 kDa) and a truncated soluble form (~ 60 kDa) (PMID: 29242513; PMID: 29725698).

实验方案

Product Specific Protocols
WB protocol for SCARF1 antibody 28174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols