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验证数据展示

  • WB analysis of COLO 320 using 24941-1-AP

    WB analysis of COLO 320 using 24941-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24941-1-AP (SCCPDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 24941-1-AP

    IP experiment of COLO 320 using 24941-1-AP

    IP result of anti-SCCPDH (IP:24941-1-AP, 3ug; Detection:24941-1-AP 1:300) with COLO 320 cells lysate 1700ug.

  • IF Staining of HepG2 using 24941-1-AP

    IF Staining of HepG2 using 24941-1-AP

    Immunofluorescent analysis of HepG2 cells using 24941-1-AP (SCCPDH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SCCPDH Polyclonal antibody

SCCPDH Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 24941-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

CGI 49, NET11, RP11 439E19.2, SCCPDH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells
Positive IP detected inCOLO 320 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24941-1-AP targets SCCPDH in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 SCCPDH fusion protein Ag11551 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 saccharopine dehydrogenase (putative)
别名 CGI 49, NET11, RP11 439E19.2, SCCPDH
计算分子量 429 aa, 47 kDa
观测分子量 40-47 kDa
GenBank蛋白编号BC026185
基因名称 SCCPDH
Gene ID (NCBI) 51097
RRIDAB_2879811
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NBX0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCCPDH antibody 24941-1-APDownload protocol
IF protocol for SCCPDH antibody 24941-1-APDownload protocol
IP protocol for SCCPDH antibody 24941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols