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SCD Polyclonal antibody
SCD Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, A375 cells, A549 cells, HepG2 cells |
Positive IHC detected in | mouse liver tissue, human liver tissue, mouse brown adipose tissue, human colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28678-1-AP targets SCD in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | SCD fusion protein Ag29156 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | stearoyl-CoA desaturase (delta-9-desaturase) |
Synonyms | Acyl CoA desaturase, Delta(9) desaturase, FADS5, Fatty acid desaturase, MSTP008, SCD, SCD1, SCDOS, Stearoyl CoA desaturase |
Calculated Molecular Weight | 355 aa, 41 kDa |
Observed Molecular Weight | 28-42 kDa |
GenBank Accession Number | BC005807 |
Gene Symbol | SCD |
Gene ID (NCBI) | 6319 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00767 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SCD antibody 28678-1-AP | Download protocol |
IHC protocol for SCD antibody 28678-1-AP | Download protocol |
IF protocol for SCD antibody 28678-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Free Radic Biol Med Zinc deficiency drives ferroptosis resistance by lactate production in esophageal squamous cell carcinoma | ||
Cells Targeting Wnt/β-Catenin Signaling Exacerbates Ferroptosis and Increases the Efficacy of Melanoma Immunotherapy via the Regulation of MITF | ||
Cancers (Basel) CircZBTB46 Protects Acute Myeloid Leukemia Cells from Ferroptotic Cell Death by Upregulating SCD | ||
Biomed Pharmacother The anti-fibrotic efficacy of adelmidrol depends on hepatic PPARγ levels | ||
Int Immunopharmacol E3 ubiquitin ligase Siah1 aggravates NAFLD through Scp2 ubiquitination |