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  • Featured Product
  • KD/KO Validated

SCD1 Polyclonal antibody, PBS Only

SCD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28678-1-PBS

Print datasheet

Synonyms

SCD, EC:1.14.19.1, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase

验证数据展示

  • WB analysis using 28678-1-AP (same clone as 28678-1-PBS)

    WB analysis using 28678-1-AP (same clone as 28678-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28678-1-AP (SCD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 28678-1-AP (same clone as 28678-1-PBS)

    IF Staining of MCF-7 using 28678-1-AP (same clone as 28678-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SCD antibody (28678-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 28678-1-AP (same clone as 28678-1-PBS)

    WB analysis of A431 using 28678-1-AP (same clone as 28678-1-PBS)

    WB result of SCD antibody (28678-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCD transfected A431 cells. This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)

    IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SCD antibody included
Planning an IHC experiment? We recommend our IHCeasy SCD Ready-To-Use IHC Kit. SCD primary antibody included.

产品信息

28678-1-PBS targets SCD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SCD1 fusion protein Ag29156 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stearoyl-CoA desaturase (delta-9-desaturase)
Synonyms SCD, EC:1.14.19.1, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase
Calculated Molecular Weight 355 aa, 41 kDa
Observed Molecular Weight28-42 kDa
GenBank Accession NumberBC005807
Gene Symbol SCD
Gene ID (NCBI) 6319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00767
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).