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SCF Polyclonal antibody

SCF Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23844-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 23844-1-AP

    WB analysis using 23844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23844-1-AP (SCF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 23844-1-AP

    IF Staining of HEK-293 using 23844-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 23844-1-AP

    IF Staining of HepG2 using 23844-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells
Positive IF/ICC detected inHepG2 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23844-1-AP targets SCF in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SCF fusion protein Ag20837 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIT ligand
Synonyms
Calculated Molecular Weight 273 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC074725
Gene Symbol SCF
Gene ID (NCBI) 4254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP21583
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis.

实验方案

Product Specific Protocols
WB protocol for SCF antibody 23844-1-APDownload protocol
IF protocol for SCF antibody 23844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols