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SCFD2 Polyclonal antibody

SCFD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13375-1-AP

Print datasheet

Synonyms

Syntaxin-binding protein 1-like 1, STXBP1L1, Sec1 family domain-containing protein 2

验证数据展示

  • WB analysis of COLO 320 using 13375-1-AP

    WB analysis of COLO 320 using 13375-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 13375-1-AP

    IP experiment of COLO 320 using 13375-1-AP

    IP result of anti-SCFD2 (IP:13375-1-AP, 4ug; Detection:13375-1-AP 1:200) with COLO 320 cells lysate 3200ug.

  • IHC staining of human colon cancer using 13375-1-AP

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 13375-1-AP

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon using 13375-1-AP

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 13375-1-AP

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOLO 320 cells, mouse kidney tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13375-1-AP targets SCFD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SCFD2 fusion protein Ag4197 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sec1 family domain containing 2
Synonyms Syntaxin-binding protein 1-like 1, STXBP1L1, Sec1 family domain-containing protein 2
Calculated Molecular Weight 684 aa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC032453
Gene Symbol SCFD2
Gene ID (NCBI) 152579
RRIDAB_10646473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WU76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCFD2 antibody 13375-1-APDownload protocol
IHC protocol for SCFD2 antibody 13375-1-APDownload protocol
IP protocol for SCFD2 antibody 13375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols