Secretogranin II Polyclonal antibody

Secretogranin II Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 20357-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (2)

CHGC, Chromogranin C, SCG2, Secretogranin 2, Secretogranin II, SgII, SN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse pancreas tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy Secretogranin II Ready-To-Use IHC Kit. Secretogranin II primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20357-1-AP targets Secretogranin II in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Secretogranin II fusion protein Ag14215 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 secretogranin II (chromogranin C)
别名 CHGC, Chromogranin C, SCG2, Secretogranin 2, Secretogranin II, SgII, SN
计算分子量 617 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC022509
基因名称 Chromogranin C/SGII
Gene ID (NCBI) 7857
RRIDAB_10699881
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13521
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCG2, also known as secretogranin II or Chromogranin C, is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is widely distributed in nervous and endocrine tissues, and abundant in neuroendocrine granules. In the brain secretogranin II is almost completely processed to secretoneurin, a peptide involved in neurite outgrowth, neuroprotection and neuronal plasticity.

实验方案

Product Specific Protocols
WB protocol for Secretogranin II antibody 20357-1-APDownload protocol
IHC protocol for Secretogranin II antibody 20357-1-APDownload protocol
IF protocol for Secretogranin II antibody 20357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Cell Rep

Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.

Authors - Tian Wang
mouse,ratWB,IF

J Cell Biol

SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.

Authors - Hongfei Xu
humanWB,IHC,CoIP

Mol Oncol

Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer.

Authors - Chao Fang
humanIHC

Front Mol Biosci

Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts.

Authors - Hufei Wang
humanIHC

Front Physiol

Potential biomarkers and immune cell infiltration involved in aortic valve calcification identified through integrated bioinformatics analysis

Authors - Xiaoshuo Lv
mouseWB

Front Neurol

Tandem mass tag-based quantitative proteomic analysis of effects of multiple sevoflurane exposures on the cerebral cortex of neonatal and adult mice

Authors - Jingyu Feng
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