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SCGN Polyclonal antibody

SCGN Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14919-1-AP

Print datasheet

Synonyms

CALBL, SCGN, SECRET, Secretagogin, SEGN, setagin

验证数据展示

  • WB analysis of mouse pancreas using 14919-1-AP

    WB analysis of mouse pancreas using 14919-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 14919-1-AP

    IP experiment of mouse brain using 14919-1-AP

    IP result of anti-SCGN (IP:14919-1-AP, 4ug; Detection:14919-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse pancreas tissue, fetal human brain tissue, mouse colon tissue, mouse liver tissue, mouse stomach tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14919-1-AP targets SCGN in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SCGN fusion protein Ag6736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secretagogin, EF-hand calcium binding protein
Synonyms CALBL, SCGN, SECRET, Secretagogin, SEGN, setagin
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000336
Gene Symbol SCGN
Gene ID (NCBI) 10590
RRIDAB_2183555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76038
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCGN antibody 14919-1-APDownload protocol
IP protocol for SCGN antibody 14919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols