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SCLY Monoclonal antibody

SCLY Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A2G7

Cat no : 67606-1-Ig

Print datasheet

Synonyms

hSCL, SCL, SCLY, selenocysteine lyase

验证数据展示

  • WB analysis using 67606-1-Ig

    WB analysis using 67606-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67606-1-Ig (SCLY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67606-1-Ig

    IHC staining of human breast cancer using 67606-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67606-1-Ig (SCLY antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells, L02 cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67606-1-Ig targets SCLY in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, rat
Cited Reactivityhuman, mouse
Immunogen SCLY fusion protein Ag28841 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name selenocysteine lyase
Synonyms hSCL, SCL, SCLY, selenocysteine lyase
Calculated Molecular Weight 49 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC007891
Gene Symbol SCLY
Gene ID (NCBI) 51540
RRIDAB_2882812
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96I15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCLY antibody 67606-1-IgDownload protocol
IHC protocol for SCLY antibody 67606-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Nat Struct Mol Biol

PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis

Authors - Hiroaki Fujita
View Article