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SCML1 Polyclonal antibody

SCML1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30257-1-AP

Print datasheet

Synonyms

SCML1

验证数据展示

  • WB analysis using 30257-1-AP

    WB analysis using 30257-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30257-1-AP (SCML1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 30257-1-AP

    WB analysis of HepG2 using 30257-1-AP

    WB result of SCML1 antibody (30257-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML1 transfected HepG2 cells.

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30257-1-AP targets SCML1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen SCML1 fusion protein Ag33003 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sex comb on midleg-like 1 (Drosophila)
Synonyms SCML1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC026159
Gene Symbol SCML1
Gene ID (NCBI) 6322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

实验方案

Product Specific Protocols
WB protocol for SCML1 antibody 30257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols