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SCN2A Polyclonal antibody

SCN2A Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27551-1-AP

Print datasheet

Synonyms

Na(v)1.2, HBSCII, HBSCI, HBSC II, HBA

验证数据展示

  • WB analysis using 27551-1-AP

    WB analysis using 27551-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27551-1-AP (SCN2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 27551-1-AP

    IF Staining of mouse brain using 27551-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SCN2A antibody (27551-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27551-1-AP targets SCN2A in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SCN2A fusion protein Ag26022 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sodium channel, voltage-gated, type II, alpha subunit
Synonyms Na(v)1.2, HBSCII, HBSCI, HBSC II, HBA
Calculated Molecular Weight 228 kDa
Observed Molecular Weight~250 kDa
GenBank Accession NumberNM_001040142
Gene Symbol SCN2A
Gene ID (NCBI) 6326
RRIDAB_3085969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99250
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Sodium Channel, Voltage-Gated, Type II, Alpha (SCN2A) gene encodes the neuronal sodium channel NaV1.2. There are two major developmentally regulated splice isoforms of NaV1.2 that use mutually exclusive copies of the fifth coding exon and differ by one amino acid at position 209: Asparagine (Asn/N) vs. Aspartic acid (Asp/D). NaV1.2 is one of several sodium channels involved in initiation and propagation of action potentials in neurons. It's widely expressed throughout the human central nervous system, but not in peripheral tissues. (PMID: 29691040)

实验方案

Product Specific Protocols
WB protocol for SCN2A antibody 27551-1-APDownload protocol
IF protocol for SCN2A antibody 27551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols